Antibacterial Activity of Certain Members of Chlorophyceae From Warangal District, Telangana State, India

Chloroform and Methanol extracts of three genera of algae (Oedogonium crispum (Hassal) Wittrock, Rhizoclonium hieroglyphicum (Ag)Kuetz. and Spirogyra biformis C-C.Jao.) from fresh water of Warangal District were tested in vitro for their antibacterial activities against two strains of gram positive bacteria (Bacillus cereus and Staphylococcus aureus) and two strains of gram negative bacteria (Klebesiella pneumonia and Samonella typhi) by the disc diffusion method. Chloroform was the best solution for extracting the effective antibacterial materials from the algae used in this investigation, with the exception of O.crispum, for which methanol was the most effective extraction solution. Chloroform extracts of fresh S.biformis and R.heiroglyphicum showed effective results against all test organisms. For control sample observations the gentamycin was used. A significant difference between in an antibacterial activity evaluated chloroform and methanol extracts of each alga under investigation was not observed. In addition, as a result of dried and fresh extract antibacterial activity comparison, it was found that all test organisms were more sensitive to fresh extracts of the algae.